Citation

Xenogeneic-Free Culture of Human Embryonic Stem Cells

Abstract | Word Stems | Keywords | Association | Citation | Similar Titles



Abstract:

In the ten years since their discovery, significant progress has been made in elucidating the mechanisms of human embryonic stem cell (hESC) expansion and differentiation. Most of this research has used murine-derived feeder cells and culture media containing bovine-derived components. As researchers move from the bench to the clinic, there is a real need for a xenogeneic-free culture system. Ideally, hESC derivation, establishment, cell banking and undifferentiatied expansion would all be done using such a system. Here we describe an affordable, cGMP xenogeneic-free serum replacement, KNOCKOUT™ SR XenoFree, that supports the growth and expansion of hESC, without contaminating murine-derived cells or bovine-derived media components. This new formulation is based on traditional KNOCKOUT™ SR, commonly used in hESC labs. All animal-derived components in KNOCKOUT™ SR have been replaced with human-derived or synthetic components, to yield a completely xenogeneic-free formulation. Our data show that KNOCKOUT™ SR XenoFree supports hESC maintenance, cryopreservation and expansion, and can be adapted to use with or without human feeder cells, by using CELLstart™, a humanized substrate for cell culture. These formulations will facilitate hESC researchers’ move from the lab bench to clinical application of hESC, by mitigating the risk of contaminating animal-derived pathogens and possible immune rejection by the patient.
Convention
Submission, Review, and Scheduling! All Academic Convention can help with all of your abstract management needs and many more. Contact us today for a quote!
Submission - Custom fields, multiple submission types, tracks, audio visual, multiple upload formats, automatic conversion to pdf.Review - Peer Review, Bulk reviewer assignment, bulk emails, ranking, z-score statistics, and multiple worksheets!
Reports - Many standard and custom reports generated while you wait. Print programs with participant indexes, event grids, and more!Scheduling - Flexible and convenient grid scheduling within rooms and buildings. Conflict checking and advanced filtering.
Communication - Bulk email tools to help your administrators send reminders and responses. Use form letters, a message center, and much more!Management - Search tools, duplicate people management, editing tools, submission transfers, many tools to manage a variety of conference management headaches!
Click here for more information.

Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


Citation:
URL: http://citation.allacademic.com/meta/p319413_index.html
Direct Link:
HTML Code:

MLA Citation:

Tilkins, Mary Lynn., Wagner, Katherine., Phommachanh, Sackamone., Schulz, Thomas., Galeano, Maria., Robins, Allan., Chase, Lucas., Rao, Mahendra. and Vemuri, Mohan. "Xenogeneic-Free Culture of Human Embryonic Stem Cells" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p319413_index.html>

APA Citation:

Tilkins, M. , Wagner, K. , Phommachanh, S. , Schulz, T. , Galeano, M. , Robins, A. , Chase, L. G., Rao, M. and Vemuri, M. , 2009-03-23 "Xenogeneic-Free Culture of Human Embryonic Stem Cells" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p319413_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: In the ten years since their discovery, significant progress has been made in elucidating the mechanisms of human embryonic stem cell (hESC) expansion and differentiation. Most of this research has used murine-derived feeder cells and culture media containing bovine-derived components. As researchers move from the bench to the clinic, there is a real need for a xenogeneic-free culture system. Ideally, hESC derivation, establishment, cell banking and undifferentiatied expansion would all be done using such a system. Here we describe an affordable, cGMP xenogeneic-free serum replacement, KNOCKOUT™ SR XenoFree, that supports the growth and expansion of hESC, without contaminating murine-derived cells or bovine-derived media components. This new formulation is based on traditional KNOCKOUT™ SR, commonly used in hESC labs. All animal-derived components in KNOCKOUT™ SR have been replaced with human-derived or synthetic components, to yield a completely xenogeneic-free formulation. Our data show that KNOCKOUT™ SR XenoFree supports hESC maintenance, cryopreservation and expansion, and can be adapted to use with or without human feeder cells, by using CELLstart™, a humanized substrate for cell culture. These formulations will facilitate hESC researchers’ move from the lab bench to clinical application of hESC, by mitigating the risk of contaminating animal-derived pathogens and possible immune rejection by the patient.


Similar Titles:
An Efficient Protocol for Generating Neural Stem Cells (NSCs) from Human Embryonic Stem Cells

Exploring Heterogeneity In Human Embryonic Stem Cell Cultures.

Single Cell Analysis of Human Embryonic Stem Cell Differentiation


 
All Academic, Inc. is your premier source for research and conference management. Visit our website, www.allacademic.com, to see how we can help you today.