Citation

Growth of Primary Cells in a Microsystem

Abstract | Word Stems | Keywords | Association | Citation | Similar Titles



Abstract:

Precise control of the stem cell microenvironment for sustaining proliferation and differentiation is important for advances in regenerative medicine. Motivated by the recent development of induced pluripotency of primary cells, this study focuses on the design and analysis of biochip-based microculture systems for growing primary cells. The growth conditions in conventional culture dishes are highly variable, however a perfusion microsystem provides a more uniform growth environment to cells. In addition to local chemical delivery, the expenditure of precious chemical reagents is significantly reduced. Only a few milliliters of growth medium are required for a week-long experiment involving parallel runs. This study characterizes the growth kinetics of primary mouse embryonic fibroblasts, which have not been cultured in a microsystem before. Primary mouse embryonic fibroblasts are more dependent on cell-secreted factors than their transformed counterparts 3T3 fibroblasts and primary human adult fibroblasts. We found that a flow rate of 10 nL/min is required to provide the renewal of nutrient and dissolved gas and avoid the removal of cell-secreted factors. Cell growth kinetics is analyzed by fluorescence immunoassays and cell counting. The results are important for the development of new culture technology for directing cell fate and tissue regeneration.
Convention
All Academic Convention can solve the abstract management needs for any association's annual meeting.
Submission - Custom fields, multiple submission types, tracks, audio visual, multiple upload formats, automatic conversion to pdf.Review - Peer Review, Bulk reviewer assignment, bulk emails, ranking, z-score statistics, and multiple worksheets!
Reports - Many standard and custom reports generated while you wait. Print programs with participant indexes, event grids, and more!Scheduling - Flexible and convenient grid scheduling within rooms and buildings. Conflict checking and advanced filtering.
Communication - Bulk email tools to help your administrators send reminders and responses. Use form letters, a message center, and much more!Management - Search tools, duplicate people management, editing tools, submission transfers, many tools to manage a variety of conference management headaches!
Click here for more information.

Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


Citation:
URL: http://citation.allacademic.com/meta/p319494_index.html
Direct Link:
HTML Code:

MLA Citation:

Villa, Max., Pope, Sara., Conover, Joanne. and Fan, Tai-Hsi. "Growth of Primary Cells in a Microsystem" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p319494_index.html>

APA Citation:

Villa, M. , Pope, S. , Conover, J. C. and Fan, T. , 2009-03-23 "Growth of Primary Cells in a Microsystem" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p319494_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Precise control of the stem cell microenvironment for sustaining proliferation and differentiation is important for advances in regenerative medicine. Motivated by the recent development of induced pluripotency of primary cells, this study focuses on the design and analysis of biochip-based microculture systems for growing primary cells. The growth conditions in conventional culture dishes are highly variable, however a perfusion microsystem provides a more uniform growth environment to cells. In addition to local chemical delivery, the expenditure of precious chemical reagents is significantly reduced. Only a few milliliters of growth medium are required for a week-long experiment involving parallel runs. This study characterizes the growth kinetics of primary mouse embryonic fibroblasts, which have not been cultured in a microsystem before. Primary mouse embryonic fibroblasts are more dependent on cell-secreted factors than their transformed counterparts 3T3 fibroblasts and primary human adult fibroblasts. We found that a flow rate of 10 nL/min is required to provide the renewal of nutrient and dissolved gas and avoid the removal of cell-secreted factors. Cell growth kinetics is analyzed by fluorescence immunoassays and cell counting. The results are important for the development of new culture technology for directing cell fate and tissue regeneration.


Similar Titles:
Over-Expression of Protein Phosphatase 5 Decreases the Growth Rate of Human Fibroblast Cells

Does quality of teaching maths and student’s sitting position explain students’ achievement growth? Evidence from primary schools in Kenya

Learning barriers associated with growth numeracy skills attained by students as they progress across lower primary school grades


 
All Academic, Inc. is your premier source for research and conference management. Visit our website, www.allacademic.com, to see how we can help you today.