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Cytocompatibility and Drug-Release Behavior of Hydroxyapatite/Poly(L-Lactide) Electrospun Scaffold for Bone Repair

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Abstract:

Cytocompatibility and Drug-Release Behavior of Hydroxyapatite/Poly(L-Lactide) Electrospun Scaffold for Bone Repair
Fei Peng, Xiaohua Yu, Mei Wei*
Department of Chemical, Materials, and Biomolecular Engineering, University of Connecticut, Storrs, Connecticut 06269, USA

Human bone is a hybrid of hydroxyapatite (HA) nanoparticles and type I collagen nanofibers, which assembles into a highly porous structure with interconnected pores. Therefore, highly porous HA/fibrous polymer scaffold has attracted much attention in recent years, and it regarded as a prospective candidate for bone tissue engineering applications.

In this project, a highly porous HA/poly(L-lactide) (PLLA) scaffold with interconnected pores was prepared by co-electrospinning needle-shaped HA nano- or microsized particles and PLLA solution into nanofibrous scaffolds at a room temperature. The HA content within the scaffolds was carefully controlled by adjusting the HA/PLLA feeding ratio between 0/100 to 40/60 (w/w) during the electrospinning process. It was further increased by applying a homogenous HA coating layer onto the surface of the electrospun scaffold by biomimetic coating. The morphology of the scaffold and the lay-out of HA particles within PLLA fibers were characterized by FESEM, TEM and XRD, respectively. The cytocompatibility of the scaffolds were evaluated by culturing rat osteosarcoma ROS17/2.8 cells on the surface of the scaffold. The cell attachment, proliferation and differentiation were examined. We also explored the possibility to use the scaffold for controlled proteins release. Bovine serum albumin was used as a model protein, and it was labeled by fluorescein isothiocyanate (FITC) for BSA release amount determination. FITC-BSA was incorporated into the scaffold by co-precipitating it with a biomimetic apatite coating onto the surfaces of the scaffold, and its release behavior was investigated by soaking the scaffold in PBS for an 8-week period.

Our FESEM observations imply that the scaffolds were constructed by HA/PLLA composite fibers with diameters between 100 nm to 1 µm. These scaffolds demonstrated 3D interconnected porous structures and the size of the pores ranged from several to tens of micrometers. Moreover, both the nanosized and the microsized needle-shaped HA particles were proved to be well aligned within the PLLA nanofibers by TEM and XRD results.

Osteoblastic cells were seeded onto the surface of the scaffold. The number of cells on different scaffolds increased as the culture time increased. It is noteworthy that more cells were found on the scaffolds incorporated with HA particles than those on pure PLLA scaffold after 7 days of culture. After 10 days, such difference became more distinct. Almost all the HA/PLLA composite scaffolds showed significantly higher ALP activities than that of the pure PLLA scaffold. These results demonstrated that the studied scaffolds have good cytocompatibility and cell signaling properties. They can be a good candidate for bone repair.

Sustained release of FITC-BSA was observed for all scaffolds. The protein incorporated into the scaffold with nano-size HA particles had a faster release than the scaffold incorporated with micro-size HA particles. Nevertheless, it was demonstrated that the biomimetic apatite coating formed on both electrospun scaffolds was an effective carrier for sustained release of proteins.
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Name: Connecticut's Stem Cell Research International Symposium
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MLA Citation:

Peng, Fei., Yu, Xiaohua. and Wei, Mei. "Cytocompatibility and Drug-Release Behavior of Hydroxyapatite/Poly(L-Lactide) Electrospun Scaffold for Bone Repair" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p319619_index.html>

APA Citation:

Peng, F. , Yu, X. and Wei, M. , 2009-03-23 "Cytocompatibility and Drug-Release Behavior of Hydroxyapatite/Poly(L-Lactide) Electrospun Scaffold for Bone Repair" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p319619_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Cytocompatibility and Drug-Release Behavior of Hydroxyapatite/Poly(L-Lactide) Electrospun Scaffold for Bone Repair
Fei Peng, Xiaohua Yu, Mei Wei*
Department of Chemical, Materials, and Biomolecular Engineering, University of Connecticut, Storrs, Connecticut 06269, USA

Human bone is a hybrid of hydroxyapatite (HA) nanoparticles and type I collagen nanofibers, which assembles into a highly porous structure with interconnected pores. Therefore, highly porous HA/fibrous polymer scaffold has attracted much attention in recent years, and it regarded as a prospective candidate for bone tissue engineering applications.

In this project, a highly porous HA/poly(L-lactide) (PLLA) scaffold with interconnected pores was prepared by co-electrospinning needle-shaped HA nano- or microsized particles and PLLA solution into nanofibrous scaffolds at a room temperature. The HA content within the scaffolds was carefully controlled by adjusting the HA/PLLA feeding ratio between 0/100 to 40/60 (w/w) during the electrospinning process. It was further increased by applying a homogenous HA coating layer onto the surface of the electrospun scaffold by biomimetic coating. The morphology of the scaffold and the lay-out of HA particles within PLLA fibers were characterized by FESEM, TEM and XRD, respectively. The cytocompatibility of the scaffolds were evaluated by culturing rat osteosarcoma ROS17/2.8 cells on the surface of the scaffold. The cell attachment, proliferation and differentiation were examined. We also explored the possibility to use the scaffold for controlled proteins release. Bovine serum albumin was used as a model protein, and it was labeled by fluorescein isothiocyanate (FITC) for BSA release amount determination. FITC-BSA was incorporated into the scaffold by co-precipitating it with a biomimetic apatite coating onto the surfaces of the scaffold, and its release behavior was investigated by soaking the scaffold in PBS for an 8-week period.

Our FESEM observations imply that the scaffolds were constructed by HA/PLLA composite fibers with diameters between 100 nm to 1 µm. These scaffolds demonstrated 3D interconnected porous structures and the size of the pores ranged from several to tens of micrometers. Moreover, both the nanosized and the microsized needle-shaped HA particles were proved to be well aligned within the PLLA nanofibers by TEM and XRD results.

Osteoblastic cells were seeded onto the surface of the scaffold. The number of cells on different scaffolds increased as the culture time increased. It is noteworthy that more cells were found on the scaffolds incorporated with HA particles than those on pure PLLA scaffold after 7 days of culture. After 10 days, such difference became more distinct. Almost all the HA/PLLA composite scaffolds showed significantly higher ALP activities than that of the pure PLLA scaffold. These results demonstrated that the studied scaffolds have good cytocompatibility and cell signaling properties. They can be a good candidate for bone repair.

Sustained release of FITC-BSA was observed for all scaffolds. The protein incorporated into the scaffold with nano-size HA particles had a faster release than the scaffold incorporated with micro-size HA particles. Nevertheless, it was demonstrated that the biomimetic apatite coating formed on both electrospun scaffolds was an effective carrier for sustained release of proteins.


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