Citation

Expression of Genes involved in Self-Renewal or Lineage Priming in Mouse Embryonic Stem Cells

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Abstract:

Janice Au-Young, SriVidya Dadi, David Keys, Jon Sherlock and Caifu Chen. Life Technologies, 850 Lincoln Centre Drive, Foster City, CA 94404.

Abstract

The expression profiles of mouse embryonic stem cells (mESCs) undergoing the transition from self-renewal to commitment were studied to identify novel embryonic stem cell markers associated with the pluripotent state. A set of 384 markers identified from mESCs undergoing differentiation was validated using a 3-step approach, including reverse transcription, multiplex preamplification, and singleplex PCR. Bulk cell populations from mESCs, differentiated embryoid bodies (mEBs) and embryos were used to prepare preamplified cDNA for 384 genes over a range of 0.1 to 100 cell equivalents. Preamplification technology makes it possible to analyze hundreds of targets with limited starting material or even single cells. The sensitivity and uniformity of the preamplification step was confirmed by comparing qPCR results for reference and target genes from cDNA vs. preamp product. Single cell profiles were comparable with results from cell populations. This approach has identified a new subset of differentially expressed mRNAs in mESCs, which can be used as biomarkers to distinguish the pluripotent state from cells that have undergone differentiation.
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Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


Citation:
URL: http://citation.allacademic.com/meta/p319717_index.html
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MLA Citation:

Au-Young, Janice. "Expression of Genes involved in Self-Renewal or Lineage Priming in Mouse Embryonic Stem Cells" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p319717_index.html>

APA Citation:

Au-Young, J. , 2009-03-23 "Expression of Genes involved in Self-Renewal or Lineage Priming in Mouse Embryonic Stem Cells" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p319717_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Janice Au-Young, SriVidya Dadi, David Keys, Jon Sherlock and Caifu Chen. Life Technologies, 850 Lincoln Centre Drive, Foster City, CA 94404.

Abstract

The expression profiles of mouse embryonic stem cells (mESCs) undergoing the transition from self-renewal to commitment were studied to identify novel embryonic stem cell markers associated with the pluripotent state. A set of 384 markers identified from mESCs undergoing differentiation was validated using a 3-step approach, including reverse transcription, multiplex preamplification, and singleplex PCR. Bulk cell populations from mESCs, differentiated embryoid bodies (mEBs) and embryos were used to prepare preamplified cDNA for 384 genes over a range of 0.1 to 100 cell equivalents. Preamplification technology makes it possible to analyze hundreds of targets with limited starting material or even single cells. The sensitivity and uniformity of the preamplification step was confirmed by comparing qPCR results for reference and target genes from cDNA vs. preamp product. Single cell profiles were comparable with results from cell populations. This approach has identified a new subset of differentially expressed mRNAs in mESCs, which can be used as biomarkers to distinguish the pluripotent state from cells that have undergone differentiation.


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