Citation

Comparison of Stem Cell Array in Ovarian Cells and CD133/CD44 Cells Purified from Human Tumors

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Abstract:

Ovarian cancer is the most lethal gynecologic cancer. Multimodality treatment results in a complete clinical remission for more than 80% of the women, but this remission is typically short-lived and most (>70%) women will ultimately succumb to a lethal recurrence of their disease. Factor(s) associated with disease recurrence not well understood. Using immunostaining and FACS analysis, we selected different cell populations from patient tumor samples using 2 different cancer stem cell markers. Stem cell pluripotency arrays were performed to compare immortalized ovarian epithelium cells (IOSE), ovarian cancer SKOV3 cells and CD133-positive/negative, CD44-positive/negative, and double positive/negative cell population purified from patient tumors. CD133+ cells had the highest expression of stemness gene CD9. IOSE and CD44+ cells had the highest expression of cellular retinoid binding protein CRABP2 level. Stemness genes NOG and PTEN were increased in IOSE and CD133+, CD44+ and double positive cell population and decreased in SKOV3 cells. Furthermore, the maintenance of pluripotency genes NANOG, POU5F1 and SOX2 had higher expression level in purified cells than that of IOSE and SKOV3 cells. In conclusion, CD133+ cells isolated from human tumors at the time of surgery had the highest expression level of stemness gene CD9 compared with other cell populations and ovarian cells. Both CD133 and CD44 positive cell population had higher level of expression of maintenance of pluripotency genes compared with cultured cells. CD133+ cells had more stemness features than other marker positive cells isolated from human tumor and cell culture in the array study. (Supported by University of Connecticut, Carole and Ray Neag Comprehensive Cancer Center Start up fund.).
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Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


Citation:
URL: http://citation.allacademic.com/meta/p364764_index.html
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MLA Citation:

Brewer, Molly., Li, Zhong., Wu, Qian., Meng, Fanliang., Sun, Guiqin., Zhang, Sui. and Zou, Changping. "Comparison of Stem Cell Array in Ovarian Cells and CD133/CD44 Cells Purified from Human Tumors" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p364764_index.html>

APA Citation:

Brewer, M. , Li, Z. , Wu, Q. , Meng, F. , Sun, G. , Zhang, S. and Zou, C. , 2009-03-23 "Comparison of Stem Cell Array in Ovarian Cells and CD133/CD44 Cells Purified from Human Tumors" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p364764_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Ovarian cancer is the most lethal gynecologic cancer. Multimodality treatment results in a complete clinical remission for more than 80% of the women, but this remission is typically short-lived and most (>70%) women will ultimately succumb to a lethal recurrence of their disease. Factor(s) associated with disease recurrence not well understood. Using immunostaining and FACS analysis, we selected different cell populations from patient tumor samples using 2 different cancer stem cell markers. Stem cell pluripotency arrays were performed to compare immortalized ovarian epithelium cells (IOSE), ovarian cancer SKOV3 cells and CD133-positive/negative, CD44-positive/negative, and double positive/negative cell population purified from patient tumors. CD133+ cells had the highest expression of stemness gene CD9. IOSE and CD44+ cells had the highest expression of cellular retinoid binding protein CRABP2 level. Stemness genes NOG and PTEN were increased in IOSE and CD133+, CD44+ and double positive cell population and decreased in SKOV3 cells. Furthermore, the maintenance of pluripotency genes NANOG, POU5F1 and SOX2 had higher expression level in purified cells than that of IOSE and SKOV3 cells. In conclusion, CD133+ cells isolated from human tumors at the time of surgery had the highest expression level of stemness gene CD9 compared with other cell populations and ovarian cells. Both CD133 and CD44 positive cell population had higher level of expression of maintenance of pluripotency genes compared with cultured cells. CD133+ cells had more stemness features than other marker positive cells isolated from human tumor and cell culture in the array study. (Supported by University of Connecticut, Carole and Ray Neag Comprehensive Cancer Center Start up fund.).


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