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Fezl Plays a Significant Role in Neural Patterning

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Abstract:

Forebrain embryonic zinc-finger like (Fezl, Fezf2, Zfp312), a transcription factor expressed by subcerebral projection neurons was previously shown to be an important player in cortical motor neuron specification. In this study we investigated the effect of Fezl on neural induction and patterning through establishing both Fezl-inducible overexpression and Fezl knockdown mouse embryonic stem cell (mESC) lines. When Fezl was overexpressed, it caused a significant increase in the induction of the telencephalic marker Bf1 (Foxg1) at both the mRNA and protein levels. In contrast, the expression of Bf1 was essentially depleted in the Fezl knockdown line. The increase in the generation of telencephalic cells from the overexpression line was further confirmed by the positive expression of the forebrain-midbrain marker Otx2 and the dorsal telencephalic marker Pax6. These cells subsequently went on to become positive for the glutamatergic marker Tbr1 and the subcerebral projection marker Ctip2. Interestingly, despite these significant changes in the neuronal subtypes, the expression of neuronal precursors Sox1 and Nestin were very similar to the controls demonstrating that neural induction was not altered by Fezl. There have been many challenges associated with creating specific types of neurons from embryonic stem cells, and obtaining an enriched population of forebrain cells has been particularly difficult. However, this study provides further insight as to the necessary factors and the potential mechanisms involved as well as further elucidating the significant role that Fezl plays in forebrain specification.
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Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


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URL: http://citation.allacademic.com/meta/p369335_index.html
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MLA Citation:

Boisvert, Erin., Wang, Zhi Bo., Zhang, Xiao-Qing., Fashoyin, Adedayo., Du, Zhong-Wei., Zhang, Su-Chun. and Li, Xue-Jun. "Fezl Plays a Significant Role in Neural Patterning" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p369335_index.html>

APA Citation:

Boisvert, E. M., Wang, Z. , Zhang, X. , Fashoyin, A. , Du, Z. , Zhang, S. and Li, X. , 2009-03-23 "Fezl Plays a Significant Role in Neural Patterning" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p369335_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Forebrain embryonic zinc-finger like (Fezl, Fezf2, Zfp312), a transcription factor expressed by subcerebral projection neurons was previously shown to be an important player in cortical motor neuron specification. In this study we investigated the effect of Fezl on neural induction and patterning through establishing both Fezl-inducible overexpression and Fezl knockdown mouse embryonic stem cell (mESC) lines. When Fezl was overexpressed, it caused a significant increase in the induction of the telencephalic marker Bf1 (Foxg1) at both the mRNA and protein levels. In contrast, the expression of Bf1 was essentially depleted in the Fezl knockdown line. The increase in the generation of telencephalic cells from the overexpression line was further confirmed by the positive expression of the forebrain-midbrain marker Otx2 and the dorsal telencephalic marker Pax6. These cells subsequently went on to become positive for the glutamatergic marker Tbr1 and the subcerebral projection marker Ctip2. Interestingly, despite these significant changes in the neuronal subtypes, the expression of neuronal precursors Sox1 and Nestin were very similar to the controls demonstrating that neural induction was not altered by Fezl. There have been many challenges associated with creating specific types of neurons from embryonic stem cells, and obtaining an enriched population of forebrain cells has been particularly difficult. However, this study provides further insight as to the necessary factors and the potential mechanisms involved as well as further elucidating the significant role that Fezl plays in forebrain specification.


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