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Pax7 fate mapping identifies novel neural crest derivatives

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Abstract:

Neural crest cells are specialized cells that contribute to multiple tissues including cardiovascular tissue, pigmented skin cells, teeth, bones and cartilage in the head, neurons in the heart and gut, and glia of the peripheral nervous system. This vast cellular potential suggests that neural crest precursors could contribute to an equally broad range of clinical applications for regenerative medicine. However, the mechanisms that afford neural crest cells such diversity is largely unknown, in part due to the lack of markers that identify them. Our laboratory has recently identified Pax7 as the earliest known marker of neural crest cells and demonstrated its requirement for neural crest development in the chick embryo.

Here we show that Pax7 expression is detected in the early mammalian neural crest region and use Pax7-cre/ROSA YFP mice to fate map Pax7-expressing cells during development. In double transgenic mice, Pax7 protein is detected in a subpopulation of YFP+ cells and their progeny after Pax7 ceases to be expressed. In addition to known neural crest derivatives, we detected YFP+ cells in chemosensory tissues not previously recognized as having neural crest contributions –the olfactory epithelium and vomeronasal organ. YFP+ cells were detected throughout the lamina propria and in a subpopulation of sustentacular cells (specialized glia) of the olfactory epithelium, but not in olfactory neurons. In contrast, YFP expression was detected in a subpopulation of neurons in the vomeronasal organ. These results suggest that Pax7-expressing precursors contribute to the mammalian neural crest and have a broader developmental potential than previously recognized.
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Association:
Name: Connecticut's Stem Cell Research International Symposium
URL:
http://stemconn.org


Citation:
URL: http://citation.allacademic.com/meta/p370469_index.html
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MLA Citation:

Murdoch, Barbara., Delconte, Casey., Yardley, Nathan., Betters, Erin. and Garcia-Castro, Martin. "Pax7 fate mapping identifies novel neural crest derivatives" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT, Mar 23, 2009 <Not Available>. 2014-11-29 <http://citation.allacademic.com/meta/p370469_index.html>

APA Citation:

Murdoch, B. , Delconte, C. , Yardley, N. , Betters, E. and Garcia-Castro, M. , 2009-03-23 "Pax7 fate mapping identifies novel neural crest derivatives" Paper presented at the annual meeting of the Connecticut's Stem Cell Research International Symposium, Omni Hotel, New Haven, CT <Not Available>. 2014-11-29 from http://citation.allacademic.com/meta/p370469_index.html

Publication Type: Poster
Review Method: Peer Reviewed
Abstract: Neural crest cells are specialized cells that contribute to multiple tissues including cardiovascular tissue, pigmented skin cells, teeth, bones and cartilage in the head, neurons in the heart and gut, and glia of the peripheral nervous system. This vast cellular potential suggests that neural crest precursors could contribute to an equally broad range of clinical applications for regenerative medicine. However, the mechanisms that afford neural crest cells such diversity is largely unknown, in part due to the lack of markers that identify them. Our laboratory has recently identified Pax7 as the earliest known marker of neural crest cells and demonstrated its requirement for neural crest development in the chick embryo.

Here we show that Pax7 expression is detected in the early mammalian neural crest region and use Pax7-cre/ROSA YFP mice to fate map Pax7-expressing cells during development. In double transgenic mice, Pax7 protein is detected in a subpopulation of YFP+ cells and their progeny after Pax7 ceases to be expressed. In addition to known neural crest derivatives, we detected YFP+ cells in chemosensory tissues not previously recognized as having neural crest contributions –the olfactory epithelium and vomeronasal organ. YFP+ cells were detected throughout the lamina propria and in a subpopulation of sustentacular cells (specialized glia) of the olfactory epithelium, but not in olfactory neurons. In contrast, YFP expression was detected in a subpopulation of neurons in the vomeronasal organ. These results suggest that Pax7-expressing precursors contribute to the mammalian neural crest and have a broader developmental potential than previously recognized.


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